Regulatory
Part:BBa_K1086000
Designed by: Ítalo Faria do Valle Group: iGEM13_UFMG_Brazil (2013-09-11)
Promoter TorCAD (sensitive to TMAO)
This part is promoter of the TorCAD operon from Escherichia coli, and its expression only happens in response to Trimethylamine N-oxide (TMAO). The TMAO is a organic compound with the formula (CH3)3NO, and it is a metabolite produced by the liver in humans, but in bacteria TMAO is used to produce energy in the absence of oxygen. The TMAO binds to TorT protein, which activates the TorS protein. The TorS protein phosphorilates the TorR transcriptional factor, which binds to TorCAD promoter, activating the transcription.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |